This project is based on the data for my ERC consolidator project that examines the effect of feralisation on the methylome, genome and transcriptome of the chicken. We have a project currently (SNIC 2022/5-577). We currently have 15X coverage from over 500 chickens, plus transcriptomes from a further 1.5k birds also now uploaded (with the SciLife lab used for data generation in several instances). We will now add a further 400 methylomes in 2024. This data is being actively analysed, however due to two post-docs leaving in 2023, the analysis was limited for the middle-end of 2023. I am now hiring two additional bioinformaticians for 2024. In addition, we have also generated a massive amount of single cell Multiome data this year (combined RNA and ATAC seq data). This data consists of 6 S4 novogene flowcells (60billion reads), and analysis is also ongoing on this project. Currently, we have clustered the data, performed eQTL mapping I am now seeking to extend the useage for this project for another year.