In hematopoiesis rare stem cells (HSC) form all the cellular parts of the blood. The HSC compartment is functionally heterogeneous, consisting of biased HSCs with different preferences to generate mature blood cells. The mechanisms underying HSC lineage bias is not well studied. Our hypothesis is that there are gene expression and epigenetic differences in different lineage-biased HSCs. Therefore, we have isolated lineage-biased HSCs to investigate the transcriptome by RNA-seq, chromatin accessibility by ATAC-seq and the histone code by ChIP-seq. Bioinformatics will be done in house with our collaborator and co-investigator Dr. Robert Månsson's group at Karolinska Institutet.