Temperature fluctuations trigger intricate molecular adaptations that allow plants to respond to an ever-changing environment. Arabidopsis thaliana mutants whose genes encode core spliceosome proteins are lethal or highly defective. We hypothesise that the splicing capacity of a plant may be affected by temperature and other environmental factors. This premise will be tested by measuring the splicing capabilities in wild-type and in splicing mutant genotypes grown at different temperatures. In order to determine if plants are capable of splicing, we will combine high-throughput strand-specific RNA sequencing and long-read single-molecule RNA sequencing to investigate the effects of temperature on splicing and transcript use. Consequently, this project proposal aims to conduct the data analysis generated by this research.