SUPR
Project Generating dense SNP data from forensic bones and teeth.
Dnr:

sens2023562

Type:

NAISS SENS

Principal Investigator:

Maja Krzewinska

Affiliation:

Stockholms universitet

Start Date:

2023-10-23

End Date:

2024-11-01

Primary Classification:

30501: Forensic Science

Webpage:

Allocation

Abstract

Genetic testing is a standard tool in forensic identification. Due to its importance and the necessity to provide reliable and reproducible evidence, especially in court cases, the field remains largely conservative in its use of novel biomolecular tools. Standard forensic approach relies heavily on STR analysis which calls for relatively good DNA preservation and absence of inhibitors. This can be challenging when dealing with remains of unknown provenance and can result in either incomplete or altogether missing genetic profiles. Simultaneously, recent years have witnessed great advances in the field of ancient DNA (aDNA) and population genomics largely driven by the boom in the Next Generation Sequencing technologies and continuous development of novel computational tools. Those improvements not only led to the advancements in aDNA but also allowed for accumulation of large-scale present day human genome reference datasets. The combination of ancient genomic data with novel tools such as imputation and IBD analyses, when merged with the right reference dataset, are robust enough to enable tracing individuals’ origins down to country and even county level in the North European contexts. In the presented project we will apply standard ancient human DNA (aDNA) genomic analyses to retrieve and analyse data from 20 bones and teeth from Swedish unsolved forensic cases. The project is a collaborative effort between the Centre for Paleogenetics and the Department for Forensic Genetics and Forensic Toxicology of the National Board of Forensic Medicine. As the provenance and the exact age of the human remains analysed is unknown, the generated data should be treated as sensitive. Within the scope of the study, we will perform numerous rounds of sequencing aiming at generating minimum 1x genome coverage from the shotgun sequencing. We will then apply traditional population genomic tools including Principal Component Analyses (PCA), Admixture, f-statistics as well as imputation and IBD analyses, utilising high resolution modern refence datasets such as 1000 Genomes Project, SweGen and UKBB datasets. The project aims to shed light on the origin and hopefully help with the identification of at least some of the remains, but it also has the overarching goal of exploring if and which of the tools currently commonly utilised in aDNA have the potential to supplement forensic analyses in the future.