Our lab is interested in stimulating regeneration in the mammalian central nervous system (CNS). We aim to uncover the principles of cell fate regulation during development and repurpose them for regeneration. We use a multidisciplinary approach that involves stem cell biology, systems biology, computational biology, molecular engineering and mouse models of disease. Using single cell multiomics, we recently discovered a latent regenerative potential in adult spinal cord stem cells and designed a targeted intervention to elicit such potential. This intervention stimulated endogenous remyelination and improved axon conduction after injury (Llorens-Bobadilla et al. Science 2020). These studies suggest that activating the right endogenous genes after injury could unlock the dormant regenerative potential of resident stem cells. The current project aims at using novel CRISPR-based tools to systematically screen and identify pro-regenerative genes in vivo.