We have collected 37 biopsy samples from Swedish cattle claws, either healthy or with one of 5 lesion stages of a disease called digital dermatitis. It is a painful infectious foot disease in dairy cattle and a leading form of lameness causing substantial economic and welfare impacts. The etiology of the disease is still under debate, but it is considered a multifactorial and polybacterial disease. However, bacteria of the genus Treponema are considered key to the pathogenesis. We would like to investigate which bacterial species, and particularly which treponemal species, that are present in lesions on Swedish cattle (and compare to healthy skin). We have also isolated bacteria from the biopsies which we will characterize and describe in a related project. We have prepared DNA from the biopsies and sent samples for amplicon sequencing at Novogene, so we have the sequencing data. Both 16S rDNA sequencing and ISR2 sequencing was performed. The ISR2 is one of two 16S-23S rDNA intergenic spacer regions and has been used to better differentiate between Treponema phylotypes (originally described in Stamm et al. 2002). SLUBI has recently helped us make an ISR2 database which can be used for the analysis of the ISR2 sequences. The 16S rDNA sequencing will, hopefully, give us an idea about which bacteria are present in the lesions on Swedish cattle claws, and the ISR2 sequences an idea about which treponemal species/phylotypes that are present.