Brucellosis is a zoonotic disease that primarily affect livestock causing abortions, stillbirth, birth of weak offspring and epididymitis and orchitis. The disease in human start with a flu like symptoms such as fever and chills, weakness, malaise and with time progress to chronic disease characterized mostly by arthritis, back pain, and spondylitis. Brucella organisms may localize in any of the human organ systems causing a wide array of clinical symptoms similar to several infectious diseases hence continually misdiagnosed and underreported. Currently, 12 Brucella species are recognized; with B. melitensis, B. abortus, B. suis and B. canis being responsible for human infection with highly varying degrees of involvement. Brucella melitensis is by far the most important cause of human brucellosis followed by B. abortus and B. suis. Several serological studies conducted in Ethiopia indicated the disease is endemic in livestock and humans were also affected by the disease. The studies were conducted in a way that only one or few livestock species were addressed. Only a few studies attempted to isolate the organism from the natural hosts (domestic animals). For this purpose, 25 Brucella spp. isolates were identified from livestock samples in Afar region, North-eastern Ethiopia. The resulting isolates were confirmed trough biochemical and PCR methods. As there is only a scanty information on the molecular diversity of Brucella species in the study area, in Ethiopia as a whole, and in the greater Horn of Africa region, this study aims at filling this gap by identifying the genomic the diversity of the isolates and find their place in global Brucella epidemiology by comparing them with global isolates. For this purpose, whole-genome sequencing was conducted on the isolates. The remaining part to achieve this objective is to carry out the necessary bioinformatics analyses using appropriate bioinformatics tools and databases.