SUPR
The genetic basis of nocturnal migratory behavior in insects - genome assembly, annotation, and transcriptome analysis of the Australian Bogong moth, Agrotis infusa.
Dnr:

NAISS 2023/22-632

Type:

NAISS Small Compute

Principal Investigator:

Eric Warrant

Affiliation:

Lunds universitet

Start Date:

2023-06-30

End Date:

2024-07-01

Primary Classification:

10608: Zoology

Webpage:

Allocation

Abstract

The Bogong moth (Agrotis infusa) is a remarkable nocturnal long-distance migratory animal and an iconic Australian species. Every year in autumn, before the start of the hot summer in their breeding grounds in Australia (stretched in an arc-like distribution across southern Queensland, western/northwestern New South Wales and western Victoria), these tiny animals embark in the billions on a long nocturnal journey to find specific caves and crevices located over a 1000 km away in the Australian Alps (in New South Wales and Victoria). Here, they cluster up to the thousands per square meter on the cave walls in order to spend the summer months in a dormant state (called aestivation). In autumn, the moths return on a reverse migration home to their breeding grounds where they mate, lay eggs and die. The following generation migrates again to the same caves and crevices the following spring without any previous knowledge of the route. In our group, we study various aspects of these moths’ biology (e.g. navigational strategies employed during migration, neural encoding of migration-relevant sensory cues, specifics of brain and eye design, population genetics). In collaboration with NGI Uppsala we are aiming to sequence the Bogong moth genome in order to shed light on the genetic basis of its nocturnal migratory behavior (NGI project numbers: NGI00554 and NGI06261). For genome assembly and annotation, we were granted long term bioinformatics support from NBIS and would like to use the Uppmax resources for this purpose. In addition, we generated RNAseq data from actively migrating as well as aestivating moths (3 different tissues: eyes, antennae, brain). With this data set, we seek to perform de novo assembly and differential gene expression analysis to reveal differences between migrating and aestivating life stages. These RNAseq data will also serve to aid the annotation of our Bogong moth reference genome.