Lifestyle, including diet, is an important modifiable factor of metabolic diseases. Healthy diet has been shown to reduce the risk of various cardiometabolic diseases (CMD). As food is consumed as a part of complex meals and dietary patterns, it is important to know how dietary patterns, especially healthy dietary patterns, could assist in prevention of CMD. However, dietary assessment remains a huge challenge in nutrition studies. Hence, there is a need to establish panels of biomarkers to reflect healthy dietary pattern, and how these panels may relate to metabolic health. In this project, we aim to find biomarkers of healthy dietary patterns, and if these biomarkers are related with the early progression markers of metabolic diseases as well as with future incidence of disease. For this purpose, we will use samples obtained from individuals participating in both the Västerbotten Intervention Program (VIP) and the Swedish Cardiopulmonary Bioimage Study (SCAPIS) in Umeå. Since 1985 , habitants in Västerbotten county are invited to participate in the ongoing VIP study when they turn 40, 50 or 60 years. To date, over 100,000 unique individuals have taken part in the clinical examinations, blood sample collection, and filled in lifestyle questionnaires. Some individuals have participated more than once and have data collected 10 years apart. During 2016-2019, 2077 of VIP participants also participated in SCAPIS, where they underwent extensive clinical examination of lungs, carotid and coronary arteries, fat deposit and liver steatosis, as well as blood chemistry, besides filling in questionnaires about health, lifestyle factors, and diet. 2030 individuals (988 men and 1062 women) have available dietary data and blood samples both from VIP and SCAPIS, which allows for prospective analysis on the changes of dietary patterns and blood metabolite profiles. This study population hence will serve as a rich source of information to discover biomarkers of healthy dietary patterns associated with early risk of developing CMD. Once such markers are obtained, correlation with dietary component will point out which food items will be responsible for the blood metabolite profile associated with lower risk of CMD. This information will provide precision prevention opportunity using dietary intervention to target especially individuals with higher risk of CMD, which may be promising to slow down progression of CMD in such at-risk individuals.