Background and Aims: As the most common glomerulonephritis globally, mainly affecting younger adults and one of the most common causes of kidney failure, IgA Nephropathy (IgAN) poses an immense impact both on the individual and on the societal level. Here, we aimed to gain further molecular insight into the high interindividual variability of the clinical picture and disease progression by linking transcriptional profiling of IgAN-kidneys to clinical and histopathological data. Method: Kidney biopsies from patients with histopathologically verified IgAN/IgA vasculitis (n=84) (IgAV) (57 males, median [range] age 39 [4-89] years) and 11 living donors (LD) (six males, median [range] age 37 [30-65] years) were manually microdissected into glomerular and tubulointerstitial fractions. RNA was extracted with RNeasy lipid tissue mini kit (Qiagen, Valencia, CA, US) and evaluated using Bioanalyzer 2100 (Agilent, Santa Clara, CA, US). mRNA purification, conversion to cDNA, fragmentation and double stranded cDNA synthesis, amplification and clean-up was performed using Illumina Stranded mRNA prep ligation protocol (Illumina Inc). Paired end RNA sequencing was performed in three different batches (Illumina Novaseq 6000).