The microbiome in cervico-vaginal fluid has been shown to reflect co-occurrence of both HPV and HIV infection in women and influence the likelihood of developing HPV-induced cervical cancer. Previously, microbiomes have been characterized by amplification of selected regions in the 16S gene followed by short-read sequencing allowing for partial identification of the bacterial species. In this project we will amplify and sequence full-length 16S genes using the long-read Pacific Biosciences instrumentation in samples collected from a large number of women. This will enable complete identification of the bacterial species and comparisons to the HPV and HIV status of the women.