Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest forms of cancer with currently no efficient drug treatments. Our lab aims at understanding the interactions between tumour cells and the surrounding microenvironment. A molecular and functional characterization of the interactions with surrounding stroma might identify novel therapeutic targets. In this project, stroma and cancer cells from human PDAC samples will be characterized using RNA-sequencing and chromatin (ATAC-seq) and mass spectrometry profiling. Since the data consists of sequence from patient source, the work involves sensitive personal data. The resource will be used for read mapping, read count and downstream statistical analyses including gene expression, isoforms and variant calling.