The goal of our research is to develop novel RNA-based treatments to improve healing of human skin wounds. The immense economic and social impact of deficient wound healing e.g. chronic ulcers post-surgical wounds care and skin scarring, calls for attention and allocation of resources to understand biological mechanisms underlying wound complications. Due to the complex nature of wounds, efficient targeted approach to enhance healing is essentially lacking today. The recent discovery of non-coding RNAs (ncRNAs) as powerful gene regulators provides hope to develop novel RNA-based treatments for a wide variety of diseases. However, the role of ncRNAs in human skin wound healing remains largely unexplored. ​The objective of our study is to reveal the role(s) of ncRNAs in skin wound healing and to explore the potential of RNA-based therapy for chronic wounds based on whole transcriptome RNA sequencing and single nuclear sequencing technology.