Systemic mastocytosis is a severe, currently incurable hematologic neoplasm, driven by the accumulation of aberrant mast cells in several tissues. The goal of the present investigation is to decipher the mast cell differentiation in normal and perturbed hematopoiesis, with the long-term aim to improve the diagnosis and treatment of systemic mastocytosis. A single-cell transcriptional landscape of human hematopoiesis will be generated using single-cell RNA sequencing of human hematopoietic cells followed by bioinformatic analysis. The raw sequencing data (6x140=840 Gb) will be processed using the Cell Ranger pipeline for 10X Genomics data and aligned with STAR for Smart-seq2 data (generating roughly 4x6x140=3.36 Tb processed data). The generated RNA sequencing data is from human primary cells, potentially constituting sensitive data.