SUPR
MAIT cell transcriptome analysis
Dnr:

sens2023540

Type:

NAISS SENS

Principal Investigator:

Johan Sandberg

Affiliation:

Karolinska Institutet

Start Date:

2023-05-22

End Date:

2024-06-01

Primary Classification:

30110: Immunology in the medical area

Allocation

  • Cygnus /proj/nobackup at UPPMAX: 1000 GiB
  • Castor /proj at UPPMAX: 1000 GiB
  • Castor /proj/nobackup at UPPMAX: 1000 GiB
  • Cygnus /proj at UPPMAX: 1000 GiB
  • Bianca at UPPMAX: 2 x 1000 core-h/month

Abstract

Mucosa-associated invariant T cells are considered as unconventional effector memory-like T cells that reside in lymphoid or non-lymphoid tissues and mount critically early immune responses against microbial as well as viral infections. Currently, our understanding of MAIT cell immunobiology is largely limited to studies focusing on human blood or murine tissues, given the scarcity and difficulty of sampling human material. We analyse human MAIT cell populations from different tissue origins . We aim to characterize MAIT cell populations by using scRNA-seq, clone-specific TCR sequencing and epitope identification (scCITE-seq) which requires computation for sequence alignment and mapping to the human reference genome. Together with secretomic data and high-dimensional flow cytometry, the multimodal analysis of MAIT cell populations will shed light on the role of MAIT cells in human tissues.