SUPR
Genomics of thermal adaptation in the seed beetle Callosobruchus maculatus
Dnr:

NAISS 2023/22-369

Type:

NAISS Small Compute

Principal Investigator:

Rike Stelkens

Affiliation:

Stockholms universitet

Start Date:

2023-04-01

End Date:

2024-04-01

Primary Classification:

10615: Evolutionary Biology

Allocation

Abstract

We will explore the repeatability of evolution to thermal adaptation at both the phenotypic and genomic level by sequencing 12 experimental evolution lines and their 6 ancestors from an evolve-and-resequence experiment using the seed beetle Callosobruchus maculatus. We will also investigate the evolution of phenotypic plasticity and its underlying genetic framework by sampling an additional 6 experimental evolution lines which alternate temperatures every generation. First, we will identify polymorphisms that contribute to differences between the thermal regimes and that have diverged from the ancestor lines, allowing us to identify candidate loci underlying thermal adaptation. We will also look for polymorphism that show significant interactions between geographic region and thermal regime, suggesting that their role in thermal adaptation is contingent on the genetic background on which they occur. Second, we will perform targeted sequencing of a candidate outlier loci to track allele frequencies in all populations from the start of the experiment (G0) throughout generations 10, 20, 40 and 70. This effort will allows us to understand allele frequency dynamics during initial responses to climate warming from standing genetic variation and the role of epistasis and starting allele frequencies in governing these dynamics.