The advanced developments in high through-out next-generation sequencing technologies and bioinformatics approaches have unveiled eukaryotic transcriptome complexity. The prevalence of non-coding transcripts on the antisense orientation of protein-coding genes pays more attention recently. While the genome-wide role for antisense non-coding transcripts is a subject of obvious interest, its sheer existence can come at a cost for genome integrity. It raises the question of what happens when RNA polymerase II (RNAPII) molecules of sense/antisense gene pairs collide with each other by the head-to-head model. In our group, we attempt to understand how transcription of sense/antisense gene pairs is coordinated at the single locus level to maintain genome integrity in Arabidopsis thaliana. Initially, for the RNA collision project, we will use high-through out genome-wide datasets to outline genome characteristics of sense/antisense gene pairs and investigate their transcription mode at a single locus via bioinformatics and cell biology approaches.