This project will look into metagenomic data from three thermophilic biogas reactors to uncover novel acetogens. Acetogens are key players in anaerobic digestion of organic material in biogas processes. To identify acetogens, formyltetrahydrofolate synthetase and acetyl-CoA synthetase/carbon monoxide dehydrogenase will be used as functional markers for the acetogenic community. Once potential acetogens are located, potential metabolic pathways will be extrapolated and looked into to determine other potential capabilities of acetogens within the biogas system.