SUPR
Investigation on the role the transcription factors SMAD3 & PITX1 in Glioblastoma
Dnr:

NAISS 2023/22-55

Type:

NAISS Small Compute

Principal Investigator:

Chaitali Chakraborty

Affiliation:

UmeƄ universitet

Start Date:

2023-02-01

End Date:

2024-02-01

Primary Classification:

30203: Cancer and Oncology

Webpage:

Allocation

Abstract

Glioblastoma (GB) is the most malignant form of primary brain tumours. We previously determined the regulatory and topological landscapes of Glioblastoma using ChIP, ATAC, HiCHIP, and we identified two main transcription factors SMAD3 and PITX1 that probably affects the 3D organization of GB. In order to do delineate their specific roles in this context, we will first perform CUT&RUN in GB lines using antibodies against PITX1 and SMAD3. Following this we plan to do RNA-seq in knockdown and over-expression lines for SMAD3 and PITX1 genes, as well as in normal astrocytes and oligodendrocytes as control lines.