The foreign body response (FBR) to commonly used materials in regenerative medicine is not well understood and remains one of the main obstacles to clinical translation of biomaterial-based therapies. In this project, we examine the spatial and temporal evolution of the tissue response to commonly used materials implemented in wound healing applications through Visium (spatial transcriptomics) and high resolution Raman mapping in a mouse wound healing model. The combination of these technologies will enable us to examine the genes, pathways, and mechanisms that underpin the evolution of the FBR to a degree that has never been examined before. This combinatorial approach will provide a deeper understanding of wound resolution and the FBR, as well as provide guidance for future biomaterial design.