We wish to study the biology of pericytes in different pathologies by transcriptional analysis by scRNAseq were we want to extract FASTQ files from BCL2 reads in 10x cell ranger. We then want to align the reads to a reference genome with e.g. cell ranger or STARsolo. (This is mainly because our sequencing facility do not map to GFP/mCherry in their supplied alignments , and we have GFP/mCherry as a reporter for a specific cell type). Furthermore we would like to run a quality assessment of the data, preferably with 10x cell ranger. We will analyse both scRNAseq analysis and snucRNAseq data.