Interest in brain pericyte biology has increased steadily in recent years, as their role in development and disease have begun to be elucidated. However, due to their anatomical location, anonymous phenotype, and the lack of specific pericyte markers, the identification and functional characterization of pericytes is endowed with significant controversy. Here, we used a novel method for the isolation of pericytes from mouse brain combined with large scale single cell RNA sequencing Our analysis will shed new light on the molecular composition of the brain pericyte, as well as its signaling pathways in the brain.