SUPR
ATAC-seq S2 cells
Dnr:

SNIC 2022/23-380

Type:

SNIC Small Storage

Principal Investigator:

Jordi Planells

Affiliation:

Stockholms universitet

Start Date:

2022-08-02

End Date:

2023-09-01

Primary Classification:

10602: Biochemistry and Molecular Biology

Allocation

Abstract

We are interested in understanding the role of chromatin-associated RNA (caRNA) in the regulation of chromatin compaction. We have already carried out RNAi experiments to deplete exosome subunits in S2 cells (Drosophila melanogaster) and we carried out RNA-seq experiments to detect caRNAs that are exosome-sensitive. In order to investigate the biological relevance of the impaired abundances of caRNAs, we have performed a ATAC-seq to correlate RNA levels with chromatin compaction genome-wide.